Our Studies

In depth:

Figure 1 shows the levels of incorporation of thymidine tritiated into unphased PBMC cells and cultured for 60 hours in the presence of a polyethylene terephthalate (PET) prism resting on the bottom of the well. Control is represented by cells grown in wells free of PET. The PET prisms were treated on the surface with the protocols indicated in the methods and identified as "A", "B" or "C".
Since the levels of incorporation are very low and the cells little or nothing proliferating, the result of this test allows us to state that none of the surface treatments gives the prisms of PET quality activating the leukocyte proliferation and that, if anything, the superficial treatment weakly reduces the proliferation to rest of the PMBCs (* p <0.01; Mann-Whitney U Test).
One of the qualities required for a material used for the production of prostheses or sanitary devices, such as catheters, is that it does not possess the properties (directly or indirectly) of leukocyte proliferation. In this regard, the surface treatments analyzed here, are compliant with this specification and do not have the capacity to activate leukocyte proliferation. If anything, the mild inhibitory action could be useful in limiting unwanted activation.


Lymphocyte activation test: this test measured the activation of CD4 + T lymphocytes present in PBMC samples obtained from healthy donors. The expression of differentiation markers was measured by cytofluorimetry and the percentage of CD4 + and the level of expression of each single brand was evaluated by bivariate analysis. The experiments were coded on PBMCs exposed only to PET prisms (with and without surface treatment) or on cells activated with OkT3.
The data indicate that the PET treated with "B" type stratification slightly reduce the proportion of activated helper lymphocytes. None of the treated surfaces has clear activity activating helper lymphocytes present in the PBMCs samples.
In all the studied conditions, a good lymphocyte activation measured as an expression of the CD69 marker is observed.
In all the studied conditions, a good lymphocyte activation measured as an expression of the CD71 marker is observed.
In all the studied conditions, a good lymphocyte activation measured as an expression of the CD25 marker is observed.
None of the surfaces treated has been shown to significantly inhibit the activation induced by treatment with OkT3. Only for the samples exposed, during the activation, to surfaces with treatment of type "B" a modest reduction of the percentage share of CD4 + CD25 + lymphocytes is observed. This decrease is not such as to be considered clinically relevant as it is part of the experimental variability.
The results indicate that the exposure to PET surfaces treated with nanometric Ag / PEO-Like states, in the absence of stimuli, slightly increases the population of Treg (regulators) CD25 + lymphocytes and that substance B demonstrates a significant activity in reducing the baseline population of CD69 + cells. Finally, the three substances demonstrate a mild inhibitory action of lymphocyte activation induced by the OkT3 stimulus.

Experimental activities on specific industrial products

URINE CATHETERS: a first prototype lot of standard urinary catheters covered with the coating was prepared in the course of international patenting, with antibacterial, non-fouling and high lubricity properties. These prototypes are currently undergoing clinical validation at an important public hospital.
CONTACT LENTINE: a first prototype batch of contact lenses was set up, including semi-rigid lens samples, 38% Polymacon soft lenses, Ortok lenses and special lenses for keratoconus. On these samples the coating was applied during international patents, with antibacterial, non-fouling and high lubricity properties; in this case, an implementation of oxygen permeability will also be tested. The prototypes are currently in the process of application validation by a leading optical center, manufacturer of special and experimental lenses, supported by the University of Molise Ophthalmology Clinic. A second prototype lot is being prepared to deepen the experimentation under way.

FILTERS FOR AIR CONDITIONERS: an experimentation is underway on filters of air conditioners used in very crowded environments, such as university classrooms.
FILTERS FOR AIR CONDITIONERS: for clean rooms or operating rooms: an experimentation similar to the previous one on filters is being organized, to which a particularly strict antibacterial guarantee is required.
OTHER INDUSTRIAL PRODUCTS: at the request of interested companies, research and technological transfer lines can be activated for a very wide range of products, as illustrated in the introductory section concerning the competences of MEDIPLASMA